A G-type lectin receptor-like kinase in Nicotiana benthamiana enhances resistance to the fungal pathogen Sclerotinia sclerotiorum by complexing with CERK1/LYK4

Abstract Fungal pathogens are among the main destructive microorganisms for crops and ecosystems worldwide, causing substantial agricultural economic losses. Plant cell surface-localized lysin motif (LysM)-containing receptor-like kinases (RLKs) or proteins (RLPs) enhance plant resistance to fungal via sensing chitin, which is a conserved component of wall. Other types RLKs also regulate chitin signaling distinct mechanisms in plants. In this study, we identified G-type lectin RLK, NbERK1, positively regulated pathogen Sclerotinia sclerotiorum model Nicotiana benthamiana . addition, LysM-RLK NbCERK1/NbLYK4 was shown mediate S. positively. Further, association chitin-induced NbCERK1-NbLYK4 found be essential perception signaling. Importantly, NbERK1 associated with association. Moreover, could induce dissociation from complex. Also, kinase activity likely resistance-enhancing NbERK1. Together, these results suggest that novel receptor complex enhances regulating